ricinus communis Search Results


fitc lectins  (Vector Laboratories)
93
Vector Laboratories fitc lectins
Fitc Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Fluorescein+labeled+Ricinus+Communis+Agglutinin+I+(RCA+I%2C+RCA120)/bio_rxiv__64898__2026__03__02__708854-220-14-16
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biotinylated lectin ricinus communis agglutinin  (Vector Laboratories)
95
Vector Laboratories biotinylated lectin ricinus communis agglutinin
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Biotinylated Lectin Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Biotinylated+Ricinus+Communis+Agglutinin+I+(RCA+I%2C+RCA120)/pm20214679-93-82-90
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biotinylated lectin ricinus communis agglutinin - by Bioz Stars, 2026-09
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lectin  (Vector Laboratories)
94
Vector Laboratories lectin
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Rhodamine+labeled+Ricinus+Communis+Agglutinin+I+(RCA+I%2C+RCA120)/pm29861385-393-11-15
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lectin - by Bioz Stars, 2026-09
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ricin  (Vector Laboratories)
93
Vector Laboratories ricin
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Ricin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Unconjugated+Ricinus+Communis+Agglutinin+II+(RCA+II%2C+RCA60%2C+ricin)/us10774358-354-0-4
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ricin - by Bioz Stars, 2026-09
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ricinus communis  (Vector Laboratories)
94
Vector Laboratories ricinus communis
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Ricinus Communis, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Unconjugated+Ricinus+Communis+Agglutinin+I+(RCA+I%2C+RCA120)/pmc08883566__2020_274720_WARD_SUPPL-12-17-20
Average 94 stars, based on 1 article reviews
ricinus communis - by Bioz Stars, 2026-09
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agarose bound ricinus communis agglutinin i rca120  (Vector Laboratories)
94
Vector Laboratories agarose bound ricinus communis agglutinin i rca120
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Agarose Bound Ricinus Communis Agglutinin I Rca120, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Agarose+bound+Ricinus+Communis+Agglutinin+I+(RCA+I%2C+RCA120)/10__1042_slash_bj2540077-46-24-46
Average 94 stars, based on 1 article reviews
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311 in cyp11b2  (Vector Laboratories)
92
Vector Laboratories 311 in cyp11b2
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
311 In Cyp11b2, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/Anti-Ricinus+Communis+Agglutinin+(RCA)+I+%26+II%2C+Unconjugated/pm41286972-155-4-41
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ricin horseradish peroxidase conjugate rca120-hrp  (EY Laboratories)
90
EY Laboratories ricin horseradish peroxidase conjugate rca120-hrp
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Ricin Horseradish Peroxidase Conjugate Rca120 Hrp, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/ricinus+communis+agglutinin++rca+i/pmc03832357-50-0-8
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ricinus communis agglutin lectin  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies ricinus communis agglutin lectin
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Ricinus Communis Agglutin Lectin, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/ricinus+communis+agglutin+lectin/pm22079878-8-139-133
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ricinus communis agglutin lectin - by Bioz Stars, 2026-09
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ricinus communis lectin 120  (Seikagaku corporation)
90
Seikagaku corporation ricinus communis lectin 120
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Ricinus Communis Lectin 120, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/ricinus+communis+lectin+120/us07709234-483-6-10
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biotin-labeled ricinus communis agglutinin  (Seikagaku corporation)
90
Seikagaku corporation biotin-labeled ricinus communis agglutinin
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Biotin Labeled Ricinus Communis Agglutinin, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/agarose+beads+conjugated+with+ricinus+communis+agglutinin++rca+/10__1128_slash_jvi__75__10__4604___4613__2001-96-5-8
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polyploid castor plants ncimb 41593 ricinus communis b2-20-4n  (NCIMB Ltd)
90
NCIMB Ltd polyploid castor plants ncimb 41593 ricinus communis b2-20-4n
Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in <t>lectin-positive</t> microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.
Polyploid Castor Plants Ncimb 41593 Ricinus Communis B2 20 4n, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ricinus+communis/castor+plant+ncimb+41593+ricinus+communis+b2+20+4n/us08946513-111-36-39
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Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in lectin-positive microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.

Journal: The European journal of neuroscience

Article Title: Expression pattern of miR-146a, an inflammation-associated microRNA, in experimental and human temporal lobe epilepsy.

doi: 10.1111/j.1460-9568.2010.07122.x

Figure Lengend Snippet: Fig. 2. In situ hybridization analysis of miR-146a expression in hippocampal tissue of control rats and after induction of SE. (A, C, E, G) Control hippocampus showing neuronal miR-146a expression in the different hippocampal subfields, including pyramidal neurons of CA3 (C; insert in C) and CA1 (E; insert in E) regions, granule cells of the dentate gyrus (DG; G) and hilar neurons (G; insert in G: high-magnification of hilar neurons). gcl, granule cell layer. (B, D, F, H) Hippocampus 1 week post-SE showing increased miR-146a expression within the different hippocampal regions, including CA3 (D; insert in D: high-magnification of CA3), CA1 (F; insert in F: high-magnification of CA1) and DG (H). miR-146a expression was observed in both neuronal and glial cells; arrows in D, F (and insert in D) indicate positive pyramidal neurons of CA3 and CA1; positive glial cells (arrowheads in D, F and insert in D) were particularly abundant in regions of prominent gliosis (CA1, CA3 and the inner molecular layer, iml, of the DG). Insert in (H) shows a high-magnification of positive glial cells in the DG (iml). (I and J) In situ hybridization analysis of miR-146a expression in the hilar region of the hippocampus 1 week (I) and 3–4 months post-SE (J) showing increased expression in glial cells (arrows; insert in I). Sections are counterstained with haematoxylin. Inserts in (J): in situ hybridization and immunohistochemistry analysis showing in (a) absence of miR-146a (red) expression in lectin-positive microglial cells (blue) and in (b) colocalization with the astroglial marker glial fibrillary acidic protein (GFAP; purple) in astrocytes. Scale bars: 1250 lm (A and B); 70 lm (C–J); 35 lm (inserts in C, D, F and G); 20 lm (insert in E); 17 lm (inserts in H and I); 11 lm (insert in J). LT, long-term, 3–4 months after SE.

Article Snippet: For the double-staining, combining immunocytochemistry with in situ hybridization, sections were first processed for immunocytochemistry as previously described (Aronica et al., 2001a, 2003) with glial fibrillary acidic protein (GFAP; polyclonal rabbit; DAKO, Glostrup, Denmark; 1 : 4000), neuronal nuclear protein (NeuN; mouse clone MAB377; Chemicon, Temecula, CA, USA; 1 : 2000), HLA-DR [anti-human leukocyte antigen (HLA)-DP, DQ, DR (mouse clone CR3 ⁄ 43); DAKO, Glostrup, Denmark; 1 : 400], CFH (polyclonal goat; Quidel, San Diego, CA, USA; 1 : 100) or the biotinylated lectin Ricinus Communis Agglutinin I (RCA 120; Vector Laboratories, Burlingame, CA, USA; 1 : 500, for the visualization of microglial cells on rat tissue), using Fast Blue B salt (St Louis, MO, USA) or Vector Blue substrate (Vector Laboratories) as chromogen.

Techniques: In Situ Hybridization, Expressing, Control, Immunohistochemistry, Marker